NGC Discover™ 100 Pro Chromatography System from Bio-Rad

Use the NGC™ Discover 100 Pro Chromatography System in labs with higher throughput needs and requiring secure method and process development for scale-up applications during purifications at the mg to g scale.

For simultaneously detecting proteins, peptides, nucleic acids, and chromogenic molecules using expanded scouting options.

Features and Benefits of the NGC Discover 100 Pro System

• Automated 100 ml/min pumps provide accurate gradients for high-resolution separation of biomolecules
• Automated injection of large sample volumes using fixed or dynamic loops
• Gradient separations at different pH values and buffer pH monitoring, for rapid method development
• Automated buffer and salt gradients with buffer blending during automated inline buffer preparation at flow rates of up to 200 ml/min, for rapid scouting of purification conditions
• Simultaneous four-wavelength detection of proteins, peptides, nucleic acids, and chromophores, with conductivity measurement and pH detection
• Fraction collection in combination with large-volume collection using a 12-port outlet valve
• Compatibility with the C-96 Autosampler, available with or without cooling, for accurate and reproducible sample injections
• Compatibility with the NGC Fraction Collector and BioFrac™ Fraction Collector for sample collection

The NGC Discover 100 Pro System includes the following modules and kits:

• 1 NGC Two-Tier Base Frame
• 1 NGC 3rd Tier Expansion Frame
• 1 NGC 4th Tier Expansion Frame
• 1 NGC Sample Pump Module
• 2 NGC F100 Pump Modules for a flow rate of 0.01–100 ml/min at 1,450 psi (100 bar, 10 MPa)
• 3 NGC Inlet Valve Modules
• 1 NGC Column Switching Valve for switching between columns and media without the need to replumb
• 1 NGC Outlet Valve Module for collecting up to 10 fractions larger than 5 ml
• 1 NGC Mixer Module and 750µl base and top assembly, includes an extension mixer barrel (5 ml)
• 1 NGC Multi-Wavelength Detector Module for simultaneous four-wavelength detection at 190–800 nm and salt gradient monitoring with integrated conductivity monitor, includes tubing and fittings and one 5 mm flow cell
• 1 NGC Sample Inject Valve Module for automated application of small sample volumes from sample loops or large sample volumes using an optional sample pump
• 1 NGC Buffer Blending Valve Module
• 1 NGC pH Valve Module, includes 1 NGC pH Probe (Ag/AgCl)
• 1 integrated system touch screen
• 1 copy of ChromLab™ Software for instrument control, data collection, and data analysis
• 1 documentation DVD containing manuals for use with NGC Systems
• 1 NGC Buffer Tray
• 1 NGC Communication Adaptor for connecting the BioFrac Fraction Collector

Applications and Uses of Medium-Pressure Chromatography Systems

• Preparative chromatography
• Protein purification and isolation

More Information

Use the module selection guide to help you decide which system is best for you.

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and applications requirements:

• Column scouting
• Injection of large sample volumes

If you prefer to customize your NGC System using our online tool, configure your personalized NGC™ Instrument.